A Simple Method to Obtain Microbial-free in vitro Moss Cultures

Developing a suitable method for moss sterilization is a crucial step for successful in vitro culture. Different chemical and physical methods have been tried by many authors for more than a century but with low axenic culture percentages. An easy and economic (no use of chemicals or antibiotics) cultivation method is developed here, which proved high success with three moss species starting from gametophores.