Hyaline droplet accumulation in kidney of rats treated with hexachloro-1:3-butadiene: influence of age, dose and time-course †

ABSTRACT: The present research investigates the occurrence of hyaline droplet (HD) accumulation related to age, dose andtime after treatment in male Wistar rats given a single i.p. injection of hexachloro-1:3-butadiene (HCBD). In the study on age,rats from 1 to 12months of age were treated with 100mgkg 1 body weight (b.w.) HCBD dose. Rats treated at 2months ofage showed a greater accumulation of HD than the other age groups; HD accumulation was not observed in 1-month-old rats.In the dose–response study, the treatment with 25, 50 and 100mgkg 1 b.w. at 2months of age caused HD accumulation inthe proximal convoluted tubule at all doses, with the 100mgkg 1 b.w. group slightly more affected. Finally, in the time-course study, rats treated with a 100mgkg 1 b.w. dose at 2months of age and sacrificed at 6, 12, 24, 48, 72 and 96hpost-dosing showed a time-related HD accumulation in terms of incidence and severity, after 6h, with a peak at 24 and48h and decreasing at 72 and 96h. The present results show that HD accumulation is an early finding, and is unrelated todose level and particularly evident in rats of 2month of age. These findings in male rats treated with HCBD emphasize theimportance of considering the age of rats at the start of a study. The more sensitive model was used in the detection of neph-rotoxic effects of chemicals. Copyright © 2011 John Wiley & Sons, Ltd.Keywords: hexachloro-1:3-butadiene; aging; hyaline droplet; time-course; nephrotoxicity