Ethamsylate (Dicynone) interference in determination of serum creatinine, uric acid, triglycerides, and cholesterol in assays involving the Trinder reaction; in vivo and in vitro.
2014
Background: The aim of our research was the quantification of
interfering properties of the haemostatic drug Dicynone®
(ethamsylate) in serum creatinine, uric acid, cholesterol, and
triglyceride assays using the Trinder reaction. Methods: Blood
from patients was collected before and 15 minutes after
administration of 500 mg Dicynone® dose i.v. and the above
mentioned analytes were quantified using Roche assays (Cobas
8000). In our in vitro experiment, we measured concentrations
of the analytes in pooled serum aliquots with final
concentrations of Dicynone® additions 0, 30, 60, 150, and 300
mg/L. Aliquots with 60 mg/L Dicynone® were also measured at 2,
6, and 8 hours after initial measurement when stored in 22°C
and 4°C for comparison. Results: Concentrations of the measured
analytes in samples from patients administered with a 500 mg
dose of Dicynone® were lower in all cases (n = 10) when
compared to values in samples taken immediately before
treatment. The in vitro samples showed that considerable
negative interference occurred even with the low concentrations
of Dicynone® additions (30 and 60 mg/L), showing the strongest
negative interference in creatinine values, followed by uric
acid, triglycerides, and cholesterol. Using in vitro samples,
we showed strong time and temperature dependence on Dicynone®
interference. Conclusions: We found and proved significant
negative interference of the drug Dicynone® (ethamsylate) in
the clinical analysis of blood using in vivo and in vitro
experiments. Furthermore, we observed a change of this effect
in serum matrix over time and at different storage
temperatures.
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