Development of a Multiplex Bead-Based Method for the Microquantitation of δ-Catenin

delta-Catenin is overexpressed in human cancers, including prostate, breast, lung, and ovarian cancers. Therefore, detection of delta-catenin level in patient specimens can be used as a diagnostic marker for the cancer screening. In laboratories, delta-catenin levels have been analyzed by western blot, which requires multiple procedures and is incapable of multiplex analysis of a target protein from a single reaction. In this study, we aimed to develop delta-catenin antibody-conjugated magnetic beads that can be used for quantitation of delta-catenin by bead-based multiplex assay. delta-catenin level from HEK293T and CWR22Rv-1 cell lysates was quantified to determine whether the antibody-conjugatedmagnetic bead can be used for the detection of trace amounts of delta-catenin. Our results indicate that analysis with 1 mug of CWR22Rv-1 and HEK293T cell lysates showed 918.67+/-103.8 and 874.33+/-37.21 MFI values, respectively. In conclusion, the results suggest that the procedure for detection of delta-catenin level through the antibody-conjugated magnetic beads provides a sensitive and costeffective solution for screening from patient specimens.