Protective effect of SOD mimetic on the apoptosis of N27 cells induced by paraquat

Purpose To determine if manganese(Ⅲ)meso-tetrakis(N,N'-diethylimidazolium-2-yl)porphyrin(MnTDM),a superoxide dismutase(SOD)mimetic,can prevent against paraquat(PQ)induced apoptosis in N27 cells.MethodsWhen cells were pretreated with MnTDM prior to PQ,the cell viability and metabolism status were measured by MTT assay.The nuclear morphological apoptosis change was evaluated by electron microscopy.To explore the protective mechanism of MnTDM in PQ-induced N27 cells,Bcl-2 and Bax protein expression were measured by immunocytochemistry.Results MnTDM significantly inhibited the paraquat-induced decreased cell viability and dopaminergic cell density depletion in the in N27 cell line by a dose-dependent manner.MnTDM promoted Bcl-2 expression,while inhibited Bax expression induced by PQ.The rate of Bcl-2/Bax was increased.ConclusionThese results indicated that pretreatment of N27 cells with MnTDM could block PQ mediated cytotoxicity and apoptosis by Bcl-2 and Bax expression.It is suggested that MnTDM may have the potential clinical therapeutic benefits for PD.