Using an Arabidopsis oligonucleotide microarray to analyze the secretory pathway genes' Response to TuMV infection in Brassica rapa

Abstract Previous evidence suggested the Turnip mosaic virus viral ribonuclear protein complex, comprising the viral RNA, moved to the plasmodesmata via the secretory pathway. We performed a global analysis of gene expression in Brassica rapa and identified a complex array of changes affecting pathogen response. Thirty-eight secretory pathway genes were identified. The quantitative real-time polymerase chain reaction results were consistent with microarray analysis. Combined with previous studies, we speculated that the secretory pathway participates in viral replication and movement. Exploring the precise function of the genes identified in this analysis will offer new insights into viral defense in B. rapa .