Secretion functional insulin glargine directly into the culture medium by overexpression intracellularly Kex2p

A method of producing a functional insulin glargine two completely folded chains, said method comprising the steps of: i) introducing a nucleic acid encoding a pro-peptide glargine and a nucleic acid encoding a protease in Pichia pastoris, obtaining Thus a host cell Pichia pastoris recombinant; wherein the protease is the endoprotease kexin (Kex2p) encoded by SEQ ID NO: 2, and wherein the coding sequence is protease under the control of a constitutive promoter or an inducible promoter; ii) co-expressing said pro-peptide and the protease in the host cell Pichia pastoris recombinant; and iii) obtaining a fully functional insulin glargine, without additional processing to yield functionally active as an insulin analog insulin glargine.