Effects of oxidative stress induced by redox-enzyme modulation on the progression stage of rat hepatocarcinogenesis

Effects of oxidative stress induced by redox-enzyme modulation on the progression stage of heptocarcinogenesis were examined by monitoring both hepatocyte injury and hepato cellular carcinoma development in F344 rats bearing preneoplastic liver nodules induced by the Cayama-Farber procedure. Redox-enzyme modulation, which included increased cytochrome P450 reductase activity induced by phenobarbital-Na (100 mg/kg, i.p. for 3 days), inibition of DT-diaphorase by dicumarol (25 mg/kg, i.p.), depletion of glutathoine by phorone (200 mg/kg, i.p.), supplementation with the Fe(III) sodium salt of EDTA (50 mg/kg, i.g.), exerted no prominent hepatocyte injury within nodules but did cause slight injury in the surrounding hepatocytes in nodule-bearing rats