6-Thioguanine (6 TG) Resistant Mutation, Chromosomal Aberrations and Sister Chromatid Exchanges (SCE's) in V 79 Cells

ICR-170(2-methoxy-6-chloro-9-(3-(ethyl-2-2 chloro ethyl)-aminopropyl amino) acridine 2HCl)-a synthetic antitumor drug was assayed for HGPRT gene locus mutation, chromosomal aberrations and sister chromatid exchanges in V79 cells system. The tested concentrations (0.1, 0.3, 1.0 and 3.0μg/ml) showed dose dependent effect. Chromosomal abnormalities were gap, break, fragment, ring chromosome, dicentric chromosome, exchange figures and endoreduplication. The present study suggests that ICR-170 is strong mutagenic, chromotoxic and supposed to be potentially carcinogenic compound.