Production of IL-Lβ and IL-6 by Adherent Spleen Cells after the Stimulation with Lipopolysaccharides from Salmonella Enteritidis Strain

AbstractThree different procedures were used to isolate lipopolysaccharides from the Salmonella enteritidis strain 477 : phenol—water extraction with ethanol precipitation (LPS l) phenol—water extraction with methanol precipitation (LPS 2) and FPLC purification (LPS 1/1). Production of interleukin-lβ (IL-1β) and interleukin-6 (IL-6) was observed in the Supernatants of adherent spleen cells of BALB/c mice after the stimulation and cultivation of the cells. The quantity of IL-1β and IL-6 depended on the method of LPS isolation. The highest level of IL-lβ was recorded at LPS 2 and of IL-6 at the stimulation of cells by means of LPS 1.