The anti-proliferative effects of RF EMF amplitude-modulated at tumor specific frequencies and mediation by calcium.

11079 Background: Experimental and clinical evidence shows that whole body administration of low-level radiofrequency electromagnetic fields, amplitude-modulated (AM RF EMF) at specific frequencies ranging from 400 Hz to 21 kHz has efficacy in advanced hepatocellular carcinoma (HCC) (Costa, de Oliveira et al, 2011). In vitro studies demonstrate that AM RF EMF treatment inhibits cancer cell growth in a tumor-specific manner (Zimmerman, Pennison et al, 2012). RNA-seq analysis of HepG2 cells treated with AM RF EMF revealed altered expression of several mRNAs belonging to the IP3/DAG signaling pathway. Given the central modulatory effect of calcium in this pathway, we hypothesized that calcium modulates the growth inhibitory effect of RF EMF. Methods: HCC and ovarian cancer cell lines as well as NOD SCID mice, carrying subcutaneous HCC xenografts, were exposed to 27.12 MHz RF EMF modulated at cancer-specific frequencies or random chose frequencies using exposure systems designed to replicate human exposure le...