High throughput screening of cytokines, chemokines and matrix metalloproteinases in wound fluid induced by mammary surgery

// Dan Wang 1, * , Kebang Hu 2, * , Ningning Gao 3 , Hao Zhang 1 , Yanlin Jiang 1 , Caigang Liu 1 , Shouyu Wang 4 , Zuowei Zhao 1 1 Breast Disease and Reconstruction Center, Breast Cancer Key Lab of Dalian, The Second Hospital of Dalian Medical University, Dalian, China 116023 2 Department of Urology, First Hospital of Jilin University, Changchun, China 130021 3 Ultrasonic Diagnosis Department, The First Hospital of China Medical University, Shenyang, China 110001 4 Department of Surgery, the first Hospital of Dalian Medical University, Dalian, China 114000 * These authors have contributed equally to this work Correspondence to: Caigang Liu, e-mail: angel-s205@163.com Shouyu Wang, e-mail: xiaomingjints@163.com Zuowei Zhao, e-mail: zuoweizhao@163.com Keywords: breast cancer, wound fluid, proliferation Received: March 30, 2015      Accepted: July 29, 2015      Published: August 10, 2015 ABSTRACT Objective : To clarify the composition of wound fluid (WF) and investigate the impact of WF on breast cancer cell lines. Methods : The proliferation and migration of WF-treated breast cancer cells MDA-MB-231 and MCF-7 were assessed with colony formation test, MTT cell proliferation test and scratch wound test. The quantitative profiles of WF were analyzed using Bio-Plex Pro kits. Results : The proliferation and migration of WF-treated breast cancer cells were significantly higher than that of untreated cells. Fifteen cytokines, 29 chemokines and 9 matrix metalloproteinases (MMPs) were assessed in WF. The concentrations of these factors were influenced by post-surgery days, neoadjuvant chemotherapy (NAC), TNM stage, pathological type and molecular subtype. The WF harvested from patients underwent NAC showed significant higher profiles of interleukin-1β (IL-1β), IL-4, IL-6, IL-17F, IL-21, IL-23, IL-25, IL-31, Interferon γ (IFNγ), CD40 ligand (CD40L), tumor necrosis factor α (TNFα), CXCL1, CXCL2, CXCL5, CCL3, CCL7 and CCL20. Conclusions : Surgery-induced WF promotes the proliferation and migration of breast cancer cells. The composition of WF is influenced by various clinical features and provides potential therapeutic targets to control local recurrence and tumor progression.