Synergistic effect of ultrasound and switchable hydrophilicity solvent promotes microalgal cell disruption and lipid extraction for biodiesel production.

Abstract To facilitate the lipid extraction from Nannochloropsis oceanica with thick cell wall using switchable hydrophilicity solvent, ultrasound-assisted N, N, N', N'-tetraethyl-1,3-propanediamine (TEPDA) was used to effectively destruct the cell wall. TEPDA cations were adsorbed on the cells via electrostatic force and formed the electron-donor-acceptor (EDA) complex with the hydroxyl groups in cellulose. This broke the hydrogen-bonding interactions between cellulose chains and stripped them from cell wall, thus reducing the cell wall thickness from 141 nm to 68.6 nm. Moreover, TEPDA cations neutralized the negatively charged phospholipid bilayers, decreasing the cell surface zeta potential from -27.5 eV to -14.1 eV. The local electrostatic equilibrium led to cell membrane leakage. The ultrasound promoted the stripping of the cellulose chains at a power intensity of 0.5 W/mL and frequency of 20 kHz, achieving the lipid extraction efficiency of 98.2% within 2 h at a volume ratio of 1:4 of wet microalgae to TEPDA.