Role of cyclin kinase inhibitor p27 in inhibition of emodin on mesangial cell proliferation induced by tumor necrosis factor-α

Objective:To investigate the role of p27 in t he inhibition of emodin on the mesangial cell (MC) proliferation induced by tumo r necrosis factor-α(TNF-α). Methods: p27 protein of MC was detec ted with western blotting analysis. The degree of MC proliferation was estimated through [ 3 H]thymidine ([ 3 H]TdR) incorporation. Different dosa ge of emodin (50 mg/L,100 mg/L) was added into MC stimulated by TNF-α. R esults: TNF-α (200 kU/L) decreased p27 level of MC cultured in serum-fr ee DMEM for 24 hours and increased[ 3 H]TdR incorporation. Emodin increase d p27 level of MC stimulated by TNF-α and decreased [ 3 H]TdR incorporat ion. The more the emodin was added, the greater the above-mentioned effect of e modin. Conclusion: The increment of p27 level maybe play an importan t role in the inhibition of emodin on MC proliferation induced by TNF-α.