Localization of sequences related to the human RAD6 DNA repair gene on mouse Chromosomes 11 and 13

Species: Mouse Locus name: Zinc finger protein 15 Locus symbol: Zfp15 Map position: Zfp15 is localized on mouse Chromosome (Chr) 4: centromere-D4Bir11-7.4 + 2.7-Zfp15-1.1 + 1.1-D4Mit4-17.0 + 3.9-D4Bir16-7.4 +_ 2.7-(D4Mit9, D4Bir20)--4.3 + 2.1-D4Bir2110.6 + 3.2-D4Mit11-5.4 + 2.4-D4Bir23-3.2 + 1.8-D4Bir24-8.5 + 2.9-(D4Mit13, Pnd, D4Bir25, D4Mit14)-l.1 + 1.1-D4Mit42-6.4 + 2.5-(Dvl, Rnr4)-telomere. Method of mapping: Zfp15 was localized by haplotype analysis of 94 progeny from an interspecific backcross, (C57BL/6J x M. spretus)F 1 x C57BL/6J [1]. Molecular reagents used for mapping: The Zfp15 probe was obtained by polymerase chain reaction amplification of a 552-bp fragment from mouse pituitary cDNA with primers designed on the basis of the rat Zfpl5 cDNA sequence [2]. The primers amplify 465 bp of coding sequence, outside of the conserved zinc finger regions, corresponding to the last 154 amino acids, and 87 bp of the 3' untranslated region. The RT-PCR product was cloned into the pGEM4Z vector and sequenced to confirm its correspondence with the rat Zfpl5 cDNA. Allele detection: A BglI polymorphism was detected in mouse genomic DNA with the Zfp15 probe, resulting in an M. spretusspecific restriction fragment of 15 kb and a common restriction fragment of 24 kb. The mapping was confirmed with a BamHI polymorphism, resulting in two M. spretus-specific restriction fragments of 8.9 and 6.6 kb and two common fragments of 18 and 5.5 kb.