A one step solution for identification of the skeletal remains

Various forensic techniques have been applied in our laboratory in an effort to improve identification process of the victims found in several mass graves in Croatia and Bosnia and Herzegovina. In the majority of analyzed bones and teeth samples we observed the presence of minimal amount (pg) of degraded human DNA mixed with high amounts of microbial DNA. Analysis of hundred different samples may be time-consuming processes. Here we described a use of the new IQ DNA isolation system (Promega, USA), which is short and efficient method. Following the DNA isolation method all samples have been analyzed by AluQuant System and amplified with either Power Plex 16 System or AmpFlSTR ProfilerTM PCR Amplification Kit. Typing of PCR products was performed on ABI Prism 310 Genetic Analyzer.