Glass-capillary chromatography in medical research

This paper deals with our experiences with glass-capillary chromatography in medical research. We reported earlier about chromatography of fatty acid methyl esters on a 50 m glass capillary, coated with FFAP. Now we have experimented with a 50 m Silar 10 C column, which gives even better results. The analysis of bile acids on 20 m columns, coated with OV-17, SE-54 or Silar-10 C yields good chromatograms and resolves the main bile acids by base line separation. Further, we are able to report the chromatography of steroid hormones extracted from human urine on a 50 m glass capillary coated with OV-101. Preliminary assessment can be made of the chromatography of the 20 major amino acids after butylation and acetylation on a 50 m Silar-10 C column. As an example of the analysis of vitamins we have chosen the chromatography of nicotinic acid and its isomer. In summary, we are able to show the wide variety of applications of the glass capillary column in medical research.