Biochemical characterization of the inoculum of Claviceps purpurea for submerged production of ergot alkaloids

Biochemical and morphological changes during the growth and development of Claviceps purpurea seed cultures under submerged conditions were established. The fall in RNA content at about the middle of the exponential phase of growth has been found to be associated with the transition from sphacelial to sclerotial growth and may be considered the major indicator of changes in the metabolism of the fungus. It may also serve as a biochemical indicator for the optimal activity of Claviceps purpurea seed cultures.