The characteristics of resting membrane potential on smooth muscle cells and endothelial cells in guinea pigs cochlea spiral artery
2012
Objective: A variety of inner ear disease is related to microcirculation disturbance of inner ear,but smooth muscle cells(SMC) and endothelial cells(EC) of the spiral modiolar artery(SMA),which is the main blood supply to the inner ear,physiological feature is not very clear.Methods: In this study,two-intracellular microelectrode recording technique and cell staining techniques to study the SMC and EC resting membrane potential characteristics and communication links between cells of SMA.Results: Study found that SMC and EC have high and low resting membrane potential state,two state of the resting membrane potential of cells to ACh and high K+ response is completely different.The different types of cells,EC-EC,SMC-SMC and SMC-EC,can simultaneously record by two-microelectrode,two cell resting membrane potential can also be a double-high RP,double-low RP and one high-and one low-RP.Experiment recorded in one high and one low-RP are the SMC-EC types,and ECs initial membrane potential are high potential,SMCs membrane potential are low initial potential.The double-high and double-low RP can be SMC-SMC or EC-EC or SMC-EC types.Conclusion: The results show that SMC and EC in the 0.3~0.5 mm range,similar type of cells have very good communication,can function together to maintain good and consistent,heterogeneous cell performance is more different.
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