Method Development and Validation of Erythrosine (E127) Using RP-HPLC Coupled With PDA Detector

A simple, precise and accurate RP-HPLC method coupled with PDA detector has been developed for the estimation of erythrosine (E127) in food stuffs and tablet dosage forms determination was preformed on phenomenex C18 Gemini column (150X4.6mm), 5μ particle size. The mobile phase, 20mM ammonium acetate buffer: Acetenetrile: Methonol in the ratio of 40:30:30 v/v/v was selected. 529nm was selected as the detection wavelength. Calibration graph was plotted showing dependence of response (peak area) on the amount chromatograph. The method has a linear range of 0.2–1 μg/ml. The slope, intercept and correlation coefficient was found to be 0.0044, 0.00757 and 0.99957 respectively.