Comparative study on determination of alpha-hydroxybutyrate dehydrogenase, lactate dehydrogenase isoenzyme LDH-1 by adsorption on DEAE Sephadex A-50 & electrophoresis.

Serum α-hydroxybutyrate dehydrogenase (HBDH) and lactate dehydrogenase isoenzyme (LDH-1) levels by two techniques viz., adsorption on DEAE Sephadex. A-50 and by electrophoresis were estimated in helathy subjects and patients with testicular tumours. The correlation coefficient between HBDH and LDH-1 by adsorption technique was found to be r=0.8. HBDH was found to have a value almost twice that of LDH-1 by adsorption and electrophoretic technique and the activity of LDH-1 obtained by wo factor of 0.557