Quick extraction method for total DNA of yeast-like fungi for nucleic acid amplification

The invention relates to the field of molecular biology of fungi, in particular to a quick extraction method for total DNA of yeast-like fungi for nucleic acid amplification. The method integrates the freeze-thaw method, the chemical cleavage method and the mechanical wall-breaking method skillfully, DNA extraction is completed within 10-20 min, and the cheap, non-toxic and efficient fungus DNA extraction method is the fastest so far. The extraction method has the advantages of quickness, sensitivity, non-toxicity, cheapness and the like and is applicable to most of the yeast-like fungi.