High-throughput sequencing reveals microbial communities in drinking water treatment sludge from six geographically distributed plants, including potentially toxic cyanobacteria and pathogens

Abstract The microbial community structures of drinking water treatment sludge (DWTS) generated for raw water (RW) from different locations and with different source types — including river water, lake water and reservoir water —were investigated using high-throughput sequencing. Because the unit operations in the six DWTPs were similar, community composition in fresh sludge may be determined by microbial community in the corresponding RW. Although Proteobacteria , Cyanobacteria , Bacteroidetes , Firmicutes , Verrucomicrobia , and Planctomycetes were the dominant phyla among the six DWTS samples, no single phylum exhibited similar abundance across all the samples, owing to differences in total phosphorus, chemical oxygen demand, Al, Fe, and chloride in RW. Three genera of potentially toxic cyanobacteria ( Planktothrix , Microcystis and Cylindrospermopsis ), and four potential pathogens ( Escherichia coli , Bacteroides ovatus , Prevotella copri and Rickettsia ) were found in sludge samples. Because proliferation of potentially toxic cyanobacteria and Rickettsia in RW was mainly affected by nutrients, while growth of Escherichia coli , Bacteroides ovatus and Prevotella copri in RW may be influenced by Fe, control of nutrients and Fe in RW is essential to decrease toxic cyanobacteria and pathogens in DWTS.