SPME-GC-MS analysis on volatile components from five different regions of Gynostemma pentaphyllum(Thunb.)

Objective:To establish a SPME-GC-MS method for qualitative and quantitative analysis of the essential oil composition of Gynostemma pentaphyllum in five areas of four provinces.Methods:The essential oil were analysed by SPME-GC-MS,and the contents were calculated with normalization method.Results:There were 67 compositions of Gynostemma pentaphyllum in five areas.The main constituents were aldehyde,ketone,alkane,alkene,aromatic hydrocarbons,alcohol and ester compounds.There were six common compositions of five samples,which were(3,7-dimethyl-1,6-octadien-3-ol),naphthalene,[(E)-4-(2,6,6-trimethyl-2-cyclohexen-1-yl)-3-buten-2-one],[(E)-6,10-dimethyl-5,9-undecadien-2-one],[(E)-4-(2,6,6-trimethyl-1-cyclohexen-1-yl)-3-buten-2-one] and(6,10,14-trimethyl-2-pentadecanone).The highest content of Pinglixian(abbr.PL) sample was 3-octanone which reached 14.93%.The highest content of Yudongcun(abbr.YDC) was [(E)-6,10-dimethyl-5,9-undecadien-2-one] and reached 10.8%,in the sample of Maguxianjing(abbr.MGXJ),the highest one was(3,7-dimethyl-1,6-octadien-3-ol) and reached 22.4%,and the highest content of Jinyunshan(abbr.JYS) and Qingchengshan(abbr.QCS) was benzaldehyde,63.16% and 38.52% respectively.Conclusion:The volatile oil between different areas of Gynostemma pentaphyllum was significant difference.