Evaluation of strategically-designed bifunctional peptide coating by QCM-D and osteoblast behaviors on titanium

Abstract Arginine-glycine-aspartic acid (RGD) peptide is the most widely employed motif as biomimetic coating on titanium (Ti) surface to accelerate osseointegration between Ti implants and bone tissue. Ti binding motif (TBP) has the ability of specifically recognizing and binding onto Ti surface via electrostatically interaction with TiO 2 layer. Here, TBP was introduced to connect RGD with different design strategies to form three chimeric dual-targeting peptides. Quartz crystal microbalance with dissipation monitoring (QCM-D) was used to monitor the real-time absorbance of these peptides on Ti plates in molecular level as well as to estimate the properties of peptide films. In addition, MC3T3-E1 cell behaviors on Ti plates coated with these peptides were evaluated in adhesion, spreading and proliferation under mechanical loading condition which simulated the Ti implants load carrying in oral cavity.