Cerebellar HSP70 Remains Elevated During Heat Stroke Recovery despite Return to Baseline Core Temperature

Heat Stroke (HS) remains a significant health threat for civilians and U.S. Armed Forces. Unknown is whether return to normal core temperature (Tc; ±0.1°C, radiotelemetry) within 2 days of HS is an accurate indicator of full recovery. Cerebellar damage is implicated in HS sequelae, but inflammatory responses in this brain region during prolonged recovery have not been investigated. We hypothesized that the cerebellum would show a differential time course of HS recovery compared to Tc during 7 days of HS recovery. C57BL/6J mice (N=3-7/group) were heated to Tc of 42.4oC and the cerebellum was extracted at 4 time points (3 hours, 1, 2, and 7 days) of recovery. Twelve common HS biomarkers were examined using rtPCR, ELISA, and Western Blot. There were no changes in any genes at 7 days, indicating normalization of expression. HSP70 protein peaked at day 1 and remained elevated through 7 days. At 3h, five cytokine genes (IL6, HSP70, TNFα, HSF-1, IL-1β) and three chemokine genes (Ccl2, Ccl3, Ccl4) were elevated, ...