Monoclonal antibody identifying whether goat milk is mixed with cow milk or not through specificity and preparation method thereof

The invention relates to a monoclonal antibody identifying whether goat milk is mixed with cow milk or not through specificity and a preparation method thereof. According to the market, the goat milk is mixed with cow milk conventionally, seconds are sold at best quality prices, and the quality of goat milk is influenced. The skimmed cow milk serves as antigen, immunized BALB / c mice is adopted, cell fusion is performed on separated spleen cells and SP2 / 0 myeloma cells, hybridoma cells secreting monoclonal antibody stably are screened, the monoclonal antibody can be used for identifying the non-whey antigen of the cow milk through specificity, and the cross reaction with the goat milk is avoided. The lower limit of the cow milk is of 0.2%, the method can be applied to development of test strips or kits, and the foundation is provided for developing test kits and other test products for testing cow milking mixed into goat milk.