The infection of various tick species with Babesia bigemina, its transmission and identification

Strains of Boophilus decoloratus and B. microplus were easily infected with a single stock of Babesia bigemina; Boophilus annulatus could be infected less easily, and it was difficult to infect Rhipicephalus evertsi. Two strains of R. appendiculatus and one strain each of R. bursa and Hyalomma anatolicum excavatum were refractory. The same stock of B. bigemina was transmitted by nymphs and adults of B. decoloratus but only by nymphs of R. evertsi. Vertical infection was not observed in R. evertsi, whereas it persisted in B. decoloratus for at least two generations. The sporokinetes in the hemolymph of R. evertsi were significantly shorter than those in the three Boophilus species.