Fifty-six isolates representing 53 species were acquired from the collections of T. E. Hinds (CO isolates) and R. W. Davidson (RWD isolates), U. S. Forest Service, Rocky Mountain Forest and Range
1981
50, 100, and 1000 ppm) was tested on three species with Chalara-like anamorphs and three other species. Subsequently, all 53 species were tested only on 100 ppm cycloheximide. The appropriate amount of cycloheximide (Sigma Chemical Co.) was added to autoclaved malt extract agar (MEA; 15 g malt extract, 15 g agar, and 970 ml distilled water). Media were dispensed into 90 mm diam plastic Petri dishes (15 ml/plate). The center of each plate was inoculated with a 4 mm diam mycelial plug from the advancing margin of a MEA-grown culture and incubated at 25 C. All treatments were replicated three times.
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