Expression of TAP1 and TNF-ain HBV-related hepatocellular carcinoma and its clinical significance

Objective To explore the association of expression of transporter associated with antigen processing（TAP1 ）, tumor necrosis factor-alpha （TNF-ot） with occurrence and development of HBV-related hepatocellular carcinoma（HCC）. Methods The expression of TAP1 and TNF-a in 35 liver carcinoma tissues, and 10 normal liver tissues were detected with immunohistochemical EnVision method,The expression and clinical significance of TAP1, TNF-a in HBV-related HCC were analyzed. Furthermore, the association of respective expression quantity with pathological typing of HCC was analyzed. Results The expression of TAP1 ,TNF-a was negative in hepatocytes of normal liver tissues. 91.4% of HCC tissues expressed TAP1. There was significantly statistical difference in expression of TAP1 between HCC tissues and the normal hepatic tissues（P 〈0.05） ;60% of HCC tissues expressed TNF-a. There was statistical difference in expression of TNF-a between HCC and the normal hepatic tissues （ P 〈 0. 05 ）, and the positive expression rate of TNF-a in well-differenciated HCC tissues was significantly higher than that in poorly-differenciated ones （P 〈 0. 05 ）. Conclusion TAPl-related MHC- I restricted antigen processing pathway was possibly normal in HBV-related HCC. The expression quantity of TNF-a was associated with the occurrence and degree of pathological differentiation of HBV-related HCC. Key words: Hepatitis B virus ;  Hepatocellular carcinoma;  Expression