Lotus tenuisWald.& Kit.: In vitro conditions for plant regeneration from protoplasts and callus of various explants

Abstract Culture conditions have been established for callus induction and growth from different explants in Lotus tenuis . Fast-growing calli from cotyledons, roots and hypocotyls were obtained on media containing 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthalenacetic acid (NAA) alone. With leaves, best results were achieved with 2,4-D plus 6-benzylaminopurine (6-BAP) or kinetin. Only those calli grown in the presence of NAA with 6-BAP or kinetin produced shoots when transferred to regeneration medium. Protoplasts were obtained from cotyledons, roots and leaves, but only cotyledon and root protoplasts were able to divide. Plant regeneration was obtained from all kinds of calli.