AN RPHPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CEFTRIAXONE SODIUM AND SULBACTAM SODIUM IN PARENTERAL DOSAGE FORM Research Article

Simple rapid, accurate, and precise isocratic liquid chromatographic method has been develop with UV detection at 230 nm is described for simultaneous determination of Ceftriaxone sodium and sulbactam sodium in parenteral by using Agilent 1200 Series HPLC. Chromatographic separations of two drugs was achieved on a C‐18 column using a mobile phase consisting of Ammonium acetate Buffer (50mm) and Methanol (90:10 % v/v) adjusted to pH 7.0 with triethylamine, in the flow rate of 1.0mL/min. The optimum separation was achieved in less than 15minutes. The developed Liquid Chromatographic method offers symmetric peak shape, good resolution and reasonable retention time for both drugs. The method was validated as per ICH guidelines. The developed method obeys beer’s law over the concentration range of 20‐100μg/mL for Ceftriaxone sodium and 10‐50 μg/mL for sulbactam sodium.