A multiplex PCR for detection of Listeria monocytogenes and its lineages.

Deepak B. Rawool,Swapnil P. Doijad,Krupali V. Poharkar,Mamta Negi,Satyajit B. Kale,Satyaveer Singh Malik,N. V. Kurkure,Trinad Chakraborty,Sukhadeo B. Barbuddhe

A multiplex PCR for detection of Listeria monocytogenes and its lineages.

2016

Deepak B. Rawool
Swapnil P. Doijad
Krupali V. Poharkar
Mamta Negi
Satyajit B. Kale
Satyaveer Singh Malik
N. V. Kurkure
Trinad Chakraborty
Sukhadeo B. Barbuddhe

Abstract A novel multiplex PCR assay was developed to identify genus Listeria , and discriminate Listeria monocytogenes and its major lineages (LI, LII, LIII). This assay is a rapid and inexpensive subtyping method for screening and characterization of L . monocytogenes .

Keywords:

Molecular biology
Microbiology
Genus Listeria
Listeria
Multiplex polymerase chain reaction
Listeria monocytogenes
Subtyping
Virology
Biology

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