Isolation, identification and cultural optimization of native bacteria isolates as a potential bioconversion agent.

Native p opulations of bacteria we re i solated from different habitats like soi l, drain and garbage and the diversity were characterized. In all habitats total c.f.u's of b acteria were varied significantly (p=0.05) and w ere almost positively correlated with the characteristics of the habitat. On the basis of cultural and m icroscopic c haracteristics and re sults of biochemical test the isolated s trains were i dentified as Bacillus sp. (SMH/2), Bacillus sp. (G LL/5), Cellulomonas sp., Micrococcus sp. and Pseudomonas sp. In this study B CDA medium was su itable for m assive growth o f Micrococcus sp. and Pseudomonas sp. and NA medium was suitable f or Bacillus sp a nd Cellulomonas sp. The o ptimum ranges of pH and temperature were 7.25 - 7.55 a nd 25 - 35 ˚C, respectively. In Micrococcus sp. and Pseudomonas sp suitable i ncubation period was 24 hours but in Bacillus sp. (S MH/2), Bacillus sp. (GLL/5) and Cellulomonas sp. i t wa s 3 6 hours. T hese selected bacteria were used for b ioconversion of m unicipal so lid waste to select the most effective strain. Bacterial suspension was found more effective then culture discs. The highest weight loss was observed 26.54% when bacterial suspension was used and i t was 15.34% higher th en control.