A computerized approach towards the estimation of binding parameters to characterize cooperative and non-cooperative multicomponent ligand-receptor interactions

Abstract A mathematical model has been developed to analyse multicomponent ligand-receptor interaction data directly from binding experiments without resorting to approximations such as linearization. This approach may be applied to analyze binding data for radioreceptor systems involving thyroid and steroid hormones, and drugs on neurotransmitters. Affinity constants, maximal binding capacity, cooperativity and nonspecific binding may be calculated for multiple binding systems.