Determination of five coumarins in Radix Glehniae by micellar electrokinetic capillary chromatography

A micellar electrokinetic capillary chromatography method with ultraviolet detection was developed for the simultaneous determination of psoralen,xanthotoxin,isoimpinellin,bergapten and scopoletin in Radix Glehniae.The separation was performed on an uncoated fused silica capillary column(50.2 cm×75 μm×40 cm) with 20 mmol·L-1 borax solution(pH 9.6) containing 16 mmol·L-1 sodium dodecylsulfate(SDS) and 15% acetonitrile as running buffer at applied voltage of 22 kV.The detection wavelength was 214 nm.The effects of concentrations of borax solution,sodium dodecylsulfate(SDS),and organic modifier,voltage,temperature on the separation and sensitivity were investigated.The five active constituents were completely separated within 7 min.The linear ranges of psoralen,xanthotoxin,isoimpinellin,bergapten and scopoletin were 9.91-82.6,37.2-162,2.23-18.6,2.73-22.3 and 2.89-20.1 mg·L-1,respectively.And the average recoveries were 98.9%,98.4%,101.3%,99.1% and 98.0%,respectively.This simple and rapid method provided a new basis for assessment on quality of Radix Glehniae.