Screening and preliminary analysis on an embryo development-related gene from promoter trapping line in rice.

Seven promoter trapping lines,which β-glucuronidase(GUS)activity were detected in embryo,were found by screening 2227 lines from an indica promoter trapping pool.Of which one line accession NO.W9154 was further analyzed.A single copy T-DNA insertion was indicated in the W9154 line by Southern blotting and its T-DNA inserted locus was located in the second intron of an unknown protein gene in chromosome 3 in rice.Analysis on the upstream regulation sequence of the trapped gene revealed that it contained seed-specific promoter elements,such as RY motif,E-box,G-box,AACA and so on,besides general promoter elements TATA-box and CAAT-box.Further analysis was conducted on expression pattern of the candidate gene,and it was found that the candidate was only expressed in embryo and stem,but not other tissues in wild type rice.This result was completely consistent with that of the GUS expression in the trapping line w9154.Taken together,the analyses indicated that the trapped gene of the line w9154 might be an embryo development-related gene in rice.