Dectection for anti-hantaviurs IgM in patient serum with silver enhanced dot immunogold fietration assay

Objective To establish a more rapid and simple method for detecting anti-Hantavirus IgM in patient's serum.Methods A silver enhanced dot immunogold filtration assay(SEDIGFA)was developed by coating the nucleocapsid protein of Hantavirus on nitrocellulose (NC) membrance,adding colloidal gold labeled sheep anti-human IgM antibody and the silver enhancer.The whole procedure can be finished Within 10 minutes.Results The coincidence rate of the results of serum samples simultaneously operated with ELISA and SEDEIGFA was 98% for anti-Hantavirus IgM Conclusions SEDIGFA is more simple and rapid for application to small medical laboratories.It can be applied for early diagnosis of hemorrhagic fever with renal syndrome(HFRS).