Development of a chemiluminescence detector for analysis of nitrite in biological samples

A new instrument for the determination of low concentrations of nitrite is presented. Nitrite reacts with I - in acidic medium to form nitric oxide that is subsequently removed from reaction solution by scrubbing with argon gas, then nitric oxide is detected by Luminol/H 2 O 2 Chemiluminescence detection. The detection limit of nitrite is 10pmol for S/N equals 3. The calibration graph is linear up to 1000 pmol. The coefficient of variation for 500pmol nitrite in samples is 4.87 percent. Analysis time for single test is 250 S. The results are in good agreement with a spectrophotometric interfering ions and has been applied to the analysis of a variety of biological samples.