Allele specific PCR for the minor Histocompatibility antigen HA‐2

The hematopoietic system restricted minor Histocompatibility antigen (mHag), HA-2, is encoded by the novel human class I Myosin gene, MYO1G, located on the short arm of chromosome 7. The HA-2 encoding region is di-allelicand comprises the HLA-A2 restricted T cell epitope YIGEVLVSV (HA-2 v ) and its allelic counterpart YIGEVLVSM (HA-2 M ). We designed a sequence specific PCR (SSP) for both HA-2 alleles. The HA-2 genomic typing results were compared with the HA-2 CTL phenotyping in three families and revealed exact correlation. The mHag HA-2 SSP can be incorporated in DNA based typing protocols.