The fabrication of hollow BSA micro/nanocapsules

Biogenic micro/nanpocapsules, comprised of bovine serum albumin (BSA), were successfully prepared by depositing BSA multilayer on SiO2 particles followed by glutaraldehyde cross-linking and the removal of templates. Silica particles with two sizes, BMS (bimodal silica) and smaller silica particles with sizes of about 3 m and 200 nm, respectively, are used. The BSA hollow microcapsules were characterized by transmission electron microscopy (TEM) and observed by confocal laser scanning microscopy (CLSM) after signed by fluorescein isothiocyanate (FITC). The as-prepared BSA hollow microcapsules are potentially valuable in biotechnology and medicament applications.