Genetic status of a AB chimeric blood group family

In order to study the genetic status of a rare chimeric family, some samples of A3B3 family were identified by sequencing of ABO gene; flow-rSSO and PCR-SSP were used to detect loci of HLA-A,B,DRB1 genes, and multiplex amplifying with fluorescence-dye were performed for 16 short tandem repeat (STR) loci. The results indicated that two individuals from A3B3 family contained more than two alleles at ABO gene, HLA-B,DRB1 and some STR loci. In conclusion, analysis of chimeric blood group by using genotyping techniques clearly demonstrating genetic status of this rare chimeric blood group promotes further elucidation of the existing state of specific genetic status.