Constitutive activation of nuclear factor -kB: preferntial homodimerization of p50 subunits in cervical carcinoma.

1. Abstract2. Introduction3. Materials and methods3.1. Tissue specimens3.2. Antibodies3.3. Immunohistochemical analysis3.4. DNA extraction and PCR detection of HPV3.5. Preparation of nuclear extract3.6. Electrophoretic mobility shift assay (EMSA)3.7. Western blotting4. Results4.1. Enhanced DNA-binding activity of NF-kB in cervical tumors: The predominant DNA binding species is the p50/p50 homodimer4.2. DNA binding activity of NF-kB and expression of p50 homodimer increased as a function of severity of the disease4.3. In-situ localization of NF-kB by immunohistochemistry: Increased nuclear translocation of p50 during progressionof cervical cancer4.4. Constitutive activation of NF-kB and increased expression of p50 homodimer is independent of Humanpapillomavirus infection5. Discussion6. Acknowledgement7. References