Development of new highly sensitive serotonin sensor based on green synthesized silver nanoparticles decorated reduced graphene oxide

Electrochemical detectionof serotonin (5-hydroxytryptamine 5-HT) is proposed for the first time using a cost effective and eco-friendly nanocomposite of AgNPs and rGO which is synthesized through in situ green reduction process using Rosmarinus leaf extract. The sythesized nanocomposites and the elaborated thin layers have been characterized using UV-Visible, FTIR , TEM and the EIS. The sensitivity of the developed sensor was performed by differential pulse voltammetry. Electrochemical detection of serotonin using AgNPs-rGO/Pt nanostructures presents a large linear range from 0.1 nM to 100 µM and a very low limit of detection of 78 pM compared to recent works reported in the literature. The innovative approach was successfully applied to the determination of serotonin in spiked artificial urine samples.