Biophenole: Steuernde Faktoren bei der lichtorientierten Chloroplastenbewegung?

Summary In an analysis of the role of phenolic compounds 1 ) for the light-oriented chloroplast movement in Mougeotia the following results were obtained: 1. The size of cytosomes (“tannin vesicles”) changes inversely proportional to the amount of the phenolic compounds in the efflux solution. 2. The efflux of phenolic compounds becomes extremely increased by application of ammonium oxalate. 3. The size of cytosomes increases by application of bioflavonoids and concentrated efflux solutions. 4. A relative constant ratio exists between the efflux rates of phenolic compounds and of calcium. 5. 5. A high amount of the total phenolic compounds (efflux and extract!) comes from mobile cytosomes (“tannin vesicles”). 6. 6. In a first crude separation-process (by molecular sieve chromatography) 6 fractions of a phenolextract were received; some of the fractions are strong inhibitors of the high-intensity light but not of the low-intensity light chloroplast movement. 7. Concentrated solutions of the phenol efflux as well as of the phenol extract inhibit chloroplast movement as well as the luciferin/luciferase reaction in experiments to determine the ATP concentration in an extract. These results encourage us to trace the hypothesis on the regulating effect of phenolic compounds in light-oriented chloroplast movements in Mougeotia .