Analysis of the composition in fructooligosaccharide by high performance liquid chromatography

: In this paper, an analytical method for determination of fructooligosaccharide by high performance liquid chromatography(HPLC) is presented. The HPLC conditions are mu-Bondapak C18 column, 300 mm x 3.9 mm i.d., water as mobile phase and refractive index detector. Monosaccharide, disaccharide, trisaccharide, tetrasaccharide, pentasaccharide, hexasaccharide etc. can be simultaneously determined in a single injection. The method has been used to determine the fructooligosaccharides in "Boai biogen". The results are satisfactory. The CVs were 0.55%-1.1% and correlation coefficients were 0.9992-1.000. The lowest detectable limit for fructooligosaccharide was in microgram level.