Surface modification of Nano-hydroxyapatite and its application to cell transfection.

Objective:To investigate the efficiency of gene transfection mediated by hydroxyapatite-polyethyleneimine(nHA-PEI 10KD) nanoparticles in cancer cells.Methods: The shape and size of HA-PEI nano-particle were observed by Transmission Electron Microscopy(TEM),The potential of nHA-PEI and HA in acid,alkaliand neutral conditions were measured by Zeta Potential Instrument.DNA binding capacity of nHAP-PEI(10KD) was detected by agarose gel electrophoresis,MTT assy was performed to evaluate the toxicity of nHAP-PEI(10KD) to HepG2 cells.The combinations of enhanced green fluorescent protein plasmid pEGFP1 and nHA-PEI were transfected into HepG2,Hela and SW620 cells,and the transfection efficiency was evaluated by Flow Cytometry.Results: nHA-PEI(10KD) are in high dispersion and the size of particle is 60-80nm.At PH of 7.2,Zeta Potential of 42.87mV,nHA-PEI transfects into experiment cells,especially in HepG2 cells,followed by Hela,SW620.The transfection efficiency of nHA-PEI was higher than PEI(10) and nHA,but lower than liposomes.Conclusions: The modified HA by PEI cation can potentiate the transfection of enhanced green fluorescent protein plasmid into HepG2 cells.HA-PEI(10KD) composite nanoparticles are expected to become an effective gene delivery vector.