Bioactive meroditerpenes and indole alkaloids from the soil fungus Neosartorya fischeri (KUFC 6344), and the marine-derived fungi Neosartorya laciniosa (KUFC 7896) and Neosartorya tsunodae (KUFC 9213)

Abstract Two new metabolites including a new aszonalenin analogue ( 1c ) and a new meroditerpene ( 3 ) were isolated, together with aszonalenin ( 1a ), acetylaszonalenin ( 1b ), 13-oxofumitremorgin B ( 2 ), aszonapyrone A ( 4b ) and helvolic acid, from the culture of the soil fungus Neosartorya fischeri (KUFC 6344). While the ethyl acetate extract of the culture of the diseased coral-derived fungus Neosartorya laciniosa (KUFC 7896) furnished aszonapyrone B ( 4a ), aszonapyrone A ( 4b ), tryptoquivaline L and 3′-(4-oxoquinazolin-3-yl) spiro[1 H -indole-3,5′-oxolane]-2,2′-dione, the ethyl acetate extract of the culture of the marine sponge-associated fungus Neosartorya tsunodae (KUFC 9213) yielded a new analogue of chevalone C ( 5 ) and helvolic acid. The structures of the new compounds were established based on 1D and 2D NMR spectral analysis as well as HR-ESIMS. Compounds 1a – c , 2 , 3 , 4a , 4b and 5 were evaluated for their in vitro growth inhibitory activity on the MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer) and A375-C5 (melanoma) cell lines by the protein binding dye SRB method.