Repeatability of 18F-FDG PET Radiomic Features in Cervical Cancer.

Knowledge of the intrinsic variability of radiomic features is essential to the proper interpretation of changes in these features over time. The primary aim of this study was to assess the test-retest repeatability of radiomic features extracted from 18F-Fluorodeoxyglucose (FDG) positron emission tomography (PET) images of cervical tumors. The impact of different image pre-processing methods was also explored. Methods: Patients with cervical cancer underwent baseline and repeat FDG PET/CT imaging within 7 days. PET images were reconstructed using 2 methods ordered subset expectation maximization (PETOSEM) or OSEM with point-spread function (PETPSF). Tumors were segmented to produce whole-tumor volumes of interest (VOIWT) and 40% isocontours (VOI40). Voxels were either left at the default size or resampled to 3 mm isotropic voxels. SUV was discretized to a fixed number of bins (32, 64, or 128). Radiomic features were extracted from both VOIs and repeatability was then assessed using Lin's concordance correlation coefficient (CCC). Results: Eleven patients were enrolled and completed the test-retest PET/CT imaging protocol. Shape, neighborhood gray-level difference matrix (NGLDM), and gray-level cooccurence matrix (GLCM) features were repeatable with mean CCC values of 0.81. Radiomic features extracted from PETOSEM images showed significantly better repeatability than features extracted from PETPSF images (P 0.96) when extracted from VOI40. Conclusion: Shape, GLCM, and NGLDM radiomic features were consistently repeatable while gray-level run length matrix (GLRLM) and gray-level zone length matrix (GLZLM) features were highly variable. Radiomic features extracted from 40% isocontours were more repeatable than features extracted from whole-tumor contours. Changes in voxel size or SUV discretization parameters typically resulted in relatively small differences in feature value, though several features were highly sensitive to these changes.