Sequence Assigning of the Growth Differentiation Factor-9 (GDF9) Gene in Markhoz Goats

This study was conducted to sequence the Growth Differentiation Factor-9 (GDF9) from Transforming Growth Factor beta (TGF$) super family in Markhoz breed of goat. This gene has vital role in ovarian follicle development, ovulation rate and fertility. Blood samples were collected from Sanandaj Markhoz goat breeding station and DNA was extracted using standard phenol chloroform method. The primers for exon 1 and 2 were designed and used for amplification of the gene fragments through the simple PCR procedure. The length of exon 1 and 2 are 463bp and 997bp, respectively. Sequence detection was performed after amplification of the gene segments. A mutation at exon 1 in base No.233 with C|A type and two mutations at exon 2, one in base No.354 with T|C type and another in base No.594 with A|C type were recognized. These mutations were documented and recorded in the NCBI gene bank with number of GU784823.